Cathepsin B Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-223-0.05mg | 0.05mg | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CTSB, CPSB, APPS
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
Background References:
- Basrur V., et al., 2003, J. Proteome Res. 2:69-79.
- Chi A., et al., 2006, J. Proteome Res. 5:3135-3144.
- Royer-Zemmour B., et al., 2008, Hum. Mol. Genet. 17:3617-3630.
- Cao L., et al., 1994, Gene 139 (2): 163?9.
Biological Activity:
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5. 0, 0. 01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg.
Buffer:
50 mM Tris, 150 mM NaCl, pH8.0
Fusion Tag:
His Tag
NCBI Gene ID #:
1508
NCBI Official Name:
cathepsin B
NCBI Official Symbol:
CTSB
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
36.7 kDa (pro-form) and 29 kDa (mature-form)
Protein Accession #:
NP_001899
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P07858