CD25 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-459-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
IL2RA, CD25, p55, IL2-RA, IL-2-RA
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
Background References:
- Leonard W.J., et al., 1985, Science 230:633-639.
- Miedel M.C., et al., 1988, Biochem. Biophys. Res. Commun. 154:372-379.
- Wang X., et al., 2005, Science 310:1159-1163.
Biological Activity:
Immobilized Human IL-2, Tag Free at 5ug/mL (100 uL/well) can bind Human IL-2 R alpha, His Tag with a linear range of 0.01-1.11 ug/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
3559
NCBI Official Name:
interleukin 2 receptor, alpha
NCBI Official Symbol:
IL2RA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
23.6 kDa
Protein Accession #:
NP_000408
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P01589