HE1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-561-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
NPC2, HE1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Niemann-Pick disease type C2 protein (NPC2) is also known as epididymal secretory protein E1 and human epididymis-specific protein 1 (HE1). NPC2 is a intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. Also,NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Furthermore,the secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
Background References:
- Liou H.L., et al., 2006, J. Biol. Chem. 281:36710-36723.
- Xu Z., et al., 2008, Biochemistry 47:11134-11143.
- Yamanashi Y., et al., 2012, Hepatology 55:953-964.
- Millat G., et al., 2005, Mol. Genet. Metab. 86:220-232.
Buffer:
PBS, pH 7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
10577
NCBI Official Name:
Niemann-Pick disease, type C2
NCBI Official Symbol:
NPC2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
16.5 kDa
Protein Accession #:
AAH02532
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P61916