Osteopontin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-569-0.1mg | 0.1mg | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SPP1, BNSP, OPN, Uropontin, Nephropontin, Osteopontin, BSP-1, ETA-1, BSPI
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Osteopontin (OPN) is also known as Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1, Nephropontin, Urinary stone protein, Uropontin, BNSP, which belongs to the osteopontin family. OPN / SPP1 is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. Full length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of the protein known as OPN-R. Osteopontin / SPP-1 is biosynthesized by a variety of tissue types. OPN is the ligand for integrin alpha-V/beta-3. OPN / SPP1 binds tightly to hydroxyapatite and appears to form an integral part of the mineralized matrix. OPN / SPP1 probably important to cell-matrix interaction. OPN / SPP1 acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Background References:
- Wang KX., et al., 2008, Cytokine Growth Factor Rev. 19 (5-6): 333?45.
- Laff?n A., et al., 1991, J. Clin. Invest. 88 (2): 546?52.
- Christensen B., et al., 2005, Biochem. J. 390:285-292.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
6696
NCBI Official Name:
secreted phosphoprotein 1
NCBI Official Symbol:
SPP1
NCBI Organism:
homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
33 kDa
Protein Accession #:
AAH07016
Purity:
>80% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P10451