PH20 Hyaluronidase Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-626-0.025mg | 0.025mg | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N ? acetyl ? beta ? D ? glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Background References:
- Lin Y., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075.
- Gmachl M., et al., 1993, FEBS Lett. 336:545-548.
- Arming S., et al., 1997, Eur. J. Biochem. 247:810-814.
Buffer:
50 mM Tris, 100 mM NaCl, pH7.0-7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
6677
NCBI Official Name:
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
NCBI Official Symbol:
SPAM1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
52.1 kDa
Protein Accession #:
AAH26163
Purity:
>92% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P38567