RENIN Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-645-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
REN, FLJ10761, Renin, angiotensinogenase
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Renin is also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), and plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through beta-1 receptor activation. Renin can bind to ATP6AP2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, renin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3' UTR. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.
Background References:
- Nguyen G, et al., 2002, J. Clin. Invest. 109 (11): 1417?27.
- Adams DJ, et al., 2003, J. Biol. Chem. 278 (45): 44894?903.
- Ram CV., 2009, Future Cardiol 5 (5): 453?65.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
5972
NCBI Official Name:
renin
NCBI Official Symbol:
REN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
43.2 kDa
Protein Accession #:
AAH33474
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P00797