UCHL1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-762-0.05mg | 0.05mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -71°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
UCHL1, PGP9.5
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
Background References:
- Choi J., et al., 2004, J. Biol. Chem. 279:13256-13264.
- Liu Y., et al., 2002, Cell 111:209-218.
- Choudhary C., et al., 2009, Science 325:834-840.
Buffer:
20mM Tris, 500mM Nacl,1mM DTT,pH 8.0
Fusion Tag:
His Tag
NCBI Gene ID #:
7345
NCBI Official Name:
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol:
UCHL1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
25.7 kDa
Protein Accession #:
NP_004172
Purity:
>92% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P09936