VEGF 165 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-773-0.05mg | 0.05mg | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF, VEGF165
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
Background References:
- Takahashi, H. and Shibuya, M., 2005, Clin Sci (Lond) 109, 227-41.
- Neufeld, G. et al., 1999, FASEB J 13, 9-22.
- Robinson, C.J. and Stringer, S.E., 2001, J Cell Sci 114, 853-65.
Biological Activity:
Immobilized Human VEGF165, His Tag at 2ug/mL (100uL/well) can bind VEGFR1/R2-Fc with a linear range of 0.1-3 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
7422
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
VEGFA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
21.1 kDa
Protein Accession #:
P15692-4
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P15692-4