FGF1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-016-0.2mg | 0.2mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
FGF1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF acidic is a potent growth factor for fibroblasts and endothelial cells. FGF acidic is involved in wound repair, angiogenesis, and development. FGF acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism. The ability of FGF acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling. There are four distinct FGF receptors and each has multiple splice variants. FGF acidic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways.
Background References:
- Powers, C.J. et al., 2000, Endocr Relat Cancer 7, 165-97.
- Prudovsky, I. et al., 2003, J Cell Sci 116, 4871-81.
- Ornitz, D.M. and Itoh, N., 2001, Genome Biol 2, REVIEWS3005.
- Mohammadi, M. et al., 2005, Curr Opin Struct Biol 15, 506-16.
Biological Activity:
The bioactivity of rh-aFGF protein was determined in a BALB/c 3T3 mouse fibroblasts cell proliferation assay. The ED50 of each lot is between 50-200 pg/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag free
NCBI Gene ID #:
2246
NCBI Official Name:
fibroblast growth factor 1 (acidic)
NCBI Official Symbol:
FGF1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
15.8 kDa
Protein Accession #:
NP_000791
Purity:
>98% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P05230