PDL1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-610-0.2mg | 0.2mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Background References:
- Dong H., et al., 1999, Nat. Med. 5:1365-1369.
- Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
- Nishimura H., et al., 2001, Trends?in?Immunology?22:265.?
Biological Activity:
Measured by its binding ability in a functional ELISA.
Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag (1ug/well) can bind Biotinylated Mouse PD-1 / PDCD1, Fc Tag, Avi Tag (Avitag™). The linear range is 0.15-2.5 ug/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
C-Fc Tag
NCBI Gene ID #:
60533
NCBI Official Name:
CD274 antigen
NCBI Official Symbol:
Cd274
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
51.4 kDa
Protein Accession #:
NP_068693
Purity:
>92% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q9EP73