PCSK9 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-583-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Background References:
- Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928?33.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
- Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454?9.
Biological Activity:
Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10μg/ml (100μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0. 1μg/ml.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
100102
NCBI Official Name:
proprotein convertase subtilisin/kexin type 9
NCBI Official Symbol:
Pcsk9
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
72 kDa
Protein Accession #:
AAH38085.1
Purity:
>97% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q80W65