5-FAM N-succinimidyl ester
| Code | Size | Price |
|---|
| CDX-C0015-M010 | 10 mg | £200.00 |
Quantity:
| CDX-C0015-M025 | 25 mg | £389.00 |
Quantity:
| CDX-C0015-M100 | 100 mg | £1,146.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
5-Carboxyfluorescein NHS ester; 5-CFSE; 5-FAM, SE
Appearance:
Light yellow to orange powder.
CAS:
92557-80-7
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)
InChiKey:
TWOKNYRPKCLMAR-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 92557-80-7. Formula: C25H15NO9. MW: 473.39. Synthetic Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 5-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Used in fluorescent small molecule preparations. Spectral data: lambdaex 494 nm| lambdaem 520 nm (Buffer pH 9.0).
MDL:
MFCD00077322
Molecular Formula:
C25H15NO9
Molecular Weight:
473.39
Package Type:
Vial
Product Description:
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 5-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Used in fluorescent small molecule preparations. Spectral data: lambdaex 494 nm| lambdaem 520 nm (Buffer pH 9.0).
Purity:
>98% (HPLC)
SMILES:
OC(=O)C1=C(C=CC(=C1)C(=O)ON1C(=O)CCC1=O)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
Solubility Chemicals:
Soluble in DMSO, methanol or dimethylformamide.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997) | (3) S. K. Lau, et al.; J. Chromatogr. A. 809, 203 (1998) | (4) D.A. Fulcher & S.W. J. Wong; Immunol. Cell Biol. 77, 559 (1999) | (5) S. Nampalli, et al.; Bioconjugate Chem. 13, 468 (2002) | (6) L. Milroy, et al.; JACS 134, 8480 (2012)
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