6-FAM N-succinimidyl ester
| Code | Size | Price |
|---|
| CDX-C0017-M010 | 10 mg | £181.00 |
Quantity:
| CDX-C0017-M025 | 25 mg | £353.00 |
Quantity:
| CDX-C0017-M100 | 100 mg | £1,036.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
6-Carboxyfluorescein NHS ester; 6-CFSE; 6-FAM, SE
Appearance:
Yellow to orange powder or solid.
CAS:
92557-81-8
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
InChiKey:
VDABVNMGKGUPEY-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 92557-81-8. Formula: C25H15NO9. MW: 473.39. Synthetic Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: lambdaex 496 nm| lambdaem 516 nm (Buffer pH 9.0).
MDL:
MFCD00077323
Molecular Formula:
C25H15NO9
Molecular Weight:
473.39
Package Type:
Vial
Product Description:
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: lambdaex 496 nm| lambdaem 516 nm (Buffer pH 9.0).
Purity:
>98% (HPLC)
SMILES:
OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(C=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(O)C=C1O2
Solubility Chemicals:
Soluble in DMSO or dimethylformamide.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997) | (3) T.S. Seo, et al.; J. Org. Chem. 68, 609 (2003) | (4) K. Tanaka, et al.; JACS 129, 5612 (2007) | (5) G.V. Los, et al.; ACS Chem. Biol. 3, 373 (2008) | (6) S. Mizukami, et al.; JACS 134, 1623 (2012)
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