Zinc-alpha-2-glycoprotein (human) TurboELISA™ Kit
| Code | Size | Price |
|---|
| AG-48B-1000-KI01 | 96 wells | £560.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
+4°C (short term) +4°C (long term).
Further Information
Alternate Names/Synonyms:
Zn-alpha-2-GP; Zn-alpha-2-Glycoprotein; ZAG; ZA2G; AZGP1; ZNGP1
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Handling Advice:
After standard reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Plate and reagents should reach room temperature before use.
Long Description:
Turbo ELISA Assay. Detects human Zinc-alpha-2-glycoprotein. Does not detect mouse Zinc-alpha-2-glycoprotein. Colorimetric assay. Sample Types: Cell Culture Supernatant, Plasma, Serum. Range: 0.9375 to 60 ng/ml. Sensitivity: 0.23 ng/ml. This assay is a less than 1 hour sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Zinc-alpha-2-glycoprotein in biological fluids. Zinc-alpha-2-glycoprotein (ZAG) was first identified in the 1960s, and it's name derives from its precipitation from human plasma upon the addition of zinc salts. ZAG has since been found in secretory epithelial cells and in a range of body fluids. ZAG is identical to a lipid mobilizing factor isolated from the urine of patients with cancer cachexia and stimulates lipolysis in in vitro and in vivo experiments. Due to its expression in, and secretion from adipocytes, ZAG is considered an adipokine. Recently, the clinical significance of ZAG has been clarified. ZAG expression in adipocytes is inversely related to fat mass, thus it is intimately involved in the maintenance of body weight in mice and humans. Epidemiological studies have uncovered an association between ZAG and plasma cholesterol. The non-synonymous single nucleotide polymorphism rs4215 in ZAG is associated with plasma cholesterol and obesity. Structurally ZAG possesses a class I major histocompatibility complex (MHC) protein fold. It is distinct from other members of this protein family in that it is soluble, rather than being anchored to plasma membranes, and it associates with prolactin inducible protein rather than beta2-microglobulin. Similar to peptide antigen-presenting class I MHC molecules, ZAG possesses an open apical groove between its alpha1 and alpha2 domain helices.
NCBI, Uniprot Number:
P25311
Other data:
TRADEMARKS - TurboELISA™ and Turbo-coated Plate™ are Trademarks of Adipogen Life Sciences.
Package Type:
Box
Product Description:
This assay is a less than 1 hour sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Zinc-alpha-2-glycoprotein in biological fluids. Zinc-alpha-2-glycoprotein (ZAG) was first identified in the 1960s, and it's name derives from its precipitation from human plasma upon the addition of zinc salts. ZAG has since been found in secretory epithelial cells and in a range of body fluids. ZAG is identical to a lipid mobilizing factor isolated from the urine of patients with cancer cachexia and stimulates lipolysis in in vitro and in vivo experiments. Due to its expression in, and secretion from adipocytes, ZAG is considered an adipokine. Recently, the clinical significance of ZAG has been clarified. ZAG expression in adipocytes is inversely related to fat mass, thus it is intimately involved in the maintenance of body weight in mice and humans. Epidemiological studies have uncovered an association between ZAG and plasma cholesterol. The non-synonymous single nucleotide polymorphism rs4215 in ZAG is associated with plasma cholesterol and obesity. Structurally ZAG possesses a class I major histocompatibility complex (MHC) protein fold. It is distinct from other members of this protein family in that it is soluble, rather than being anchored to plasma membranes, and it associates with prolactin inducible protein rather than beta2-microglobulin. Similar to peptide antigen-presenting class I MHC molecules, ZAG possesses an open apical groove between its alpha1 and alpha2 domain helices.
Range:
0.9375 to 60 ng/ml
Sample Type:
Cell Culture Supernatant, Plasma, Serum
Sensitivity:
0.23 ng/ml
Specificity:
Detects human Zinc-alpha-2-glycoprotein. Does not detect mouse Zinc-alpha-2-glycoprotein.
Transportation:
Non-hazardous
UNSPSC Category:
ELISA Kits
UNSPSC Number:
41116126
Use & Stability:
12 months after the day of manufacturing. See expiry date on ELISA Kit box.
References
Development of a Dynamic Multi-Protein Signature of Postoperative Delirium: S.M. Vasunilashorn, et al.; The Journals of Gerontology: Series A, gly036 (2018)