FLT3 Ligand (human) (rec.) (His)
| Code | Size | Price |
|---|
| CHI-HF-201FLT3L-C010 | 10 ug | £235.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Application: Activity Assay
Shipping:
BI
Storage:
+4°C(Short Term), -20°C(Long Term)
Documents
Further Information
Alternate Names/Synonyms:
FLT3L; FMS-like Tyrosine Kinase 3 Ligand
Biological Activity:
The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
EClass:
32160000
Endotoxin:
<0.001EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized from 0.2µm-filtered solution in PBS.
Handling Advice:
Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Human FLT3L (aa 27 -184) is fused at the C-terminus to a His-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized from 0.2µm-filtered solution in PBS. Purity: >95% (SDS-PAGE). FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It binds to FLT3 (CD135) which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability increase in DC numbers.
Molecular Weight:
~25kDa (SDS-PAGE)
NCBI, Uniprot Number:
AAI26294.1
Package Type:
Plastic Vial
Product Description:
FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It binds to FLT3 (CD135) which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability increase in DC numbers.
Purity:
>95% (SDS-PAGE)
Sequence:
Human FLT3L (aa 27 -184) is fused at the C-terminus to a His-tag.
Source / Host:
HEK 293 cells
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.