DIS3L2 Antibody

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ProSci
Product Code: PSI-8347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8347-0.02mg0.02mg£150.00
Quantity:
PSI-8347-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
DIS3L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.
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Immunohistochemistry of DIS3L2 in rat brain tissue with DIS3L2 antibody at 5 μg/mL.
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Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.
Immunohistochemistry of DIS3L2 in rat brain tissue with DIS3L2 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
DIS3 like 3'-5' exoribonuclease 2, FAM6A, PRLMNS
Application Note:
DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
The exosome is involved in a multitude of cellular RNA processing and degradation events (1). DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2). DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3). DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4). DIS3L2 mutations have also been associated with sporadic Wilms tumors (3).
Background References:
  • Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64.
  • Brouwer R, Allmang C, Raijmakers R, et al. Three novel components of the human exosome. J. Biol. Chem. 2001; 276:6177-84.
  • Astuti D, Morris MR, Cooper WN, et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat. Genet. 2012; 44:277-84.
  • Malecki M, Viegas SC, Carneiro T, et al. The exonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway. EMBO J. 2013; 32:1842-54.
Buffer:
DIS3L2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2.

The immunogen is located within amino acids 720 - 770 of DIS3L2.
NCBI Gene ID #:
129563
NCBI Official Name:
DIS3 like 3'-5' exoribonuclease 2
NCBI Official Symbol:
DIS3L2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 97 kDa

Observed: 94 kDa
Protein Accession #:
NP_689596
Protein GI Number:
134288890
Purification:
DIS3L2 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.
Swissprot #:
Q8IYB7
User NOte:
Optimal dilutions for each application to be determined by the researcher.