Chondroadherin Antibody
| Code | Size | Price |
|---|
| PSI-8421-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8421-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Chondroadherin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Cartilage leucine-rich protein, CHAD, SLRR4A
Application Note:
Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).
Background References:
- Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.
- Haglund L, Tillgren V, Addis L, et al. Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment. J. Biol. Chem. 2011; 286:3925-34.
- Mansson B, Wenglen C, Morgelin M, et al. Association of chondroadherin with collagen type II. J. Biol. Chem. 2001; 276:32883-8.
- Camper L, Heinegard D, and Lundgren-Akerlund E. Integrin alpha2beta1 is a receptor for the cartilage matrix protein chondroadherin. J. Cell Biol. 1997; 138:1159-67.
Buffer:
Chondroadherin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin.
The immunogen is located within the last 50 amino acids of Chondroadherin.
The immunogen is located within the last 50 amino acids of Chondroadherin.
NCBI Gene ID #:
1101
NCBI Official Name:
Chondroadherin
NCBI Official Symbol:
CHAD
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 38 kDa
Observed: 36 kDa
Observed: 36 kDa
Protein Accession #:
NP_001258
Protein GI Number:
153251229
Purification:
Chondroadherin antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Homeostasis
SPECIFICITY:
Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
Swissprot #:
O15335
User NOte:
Optimal dilutions for each application to be determined by the researcher.