CHADL Antibody

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ProSci
Product Code: PSI-8423
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8423-0.02mg0.02mg£150.00
Quantity:
PSI-8423-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
CHADL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

1 / 4
Western blot analysis of CHADL in mouse kidney tissue lysate with CHADL antibody at (A) 1 and (B) 2 μg/ml.
2 / 4
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
3 / 4
Immunofluorescence of CHADL in mouse skeletal muscle tissue with CHADL antibody at 20 μg/mL.<br><br>Red: CHADL Antibody (8423) <br> Blue: DAPI staining
4 / 4
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
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Western blot analysis of CHADL in mouse kidney tissue lysate with CHADL antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
Immunofluorescence of CHADL in mouse skeletal muscle tissue with CHADL antibody at 20 μg/mL.<br><br>Red: CHADL Antibody (8423) <br> Blue: DAPI staining
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
Chondoadherin-like protein, SLRR4B
Application Note:
CHADL antibody can be used for the detection of CHADL by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The Chondroadherin-like (CHADL) protein, also known as SLRR4B, is homologous to the Chondroadherin protein and is expressed cartillaginous tissues (1). Like Chondroadherin, CHADL belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). CHADL is thought to play a negative regualtory role in collagen fibrillogenesis and chondrocyte differentiation (1).
Background References:
  • Tillgren V, Ho JC, Onnerfjord P, et al. The novel small leucine-rich protein chondroadherin-like (CHADL) is expressed in cartilage and modulates chondrocyte differentitation. J. Biol. Chem. 2015; 290:918-25.
  • Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.
Buffer:
CHADL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CHADL antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human CHADL.

The immunogen is located within the last 50 amino acids of CHADL.
NCBI Gene ID #:
150356
NCBI Official Name:
chondroadherin-like
NCBI Official Symbol:
CHADL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 84 kDa

Observed: 86 kDa
Protein Accession #:
NP_612490
Protein GI Number:
194440719
Purification:
CHADL Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell, Homeostasis
SPECIFICITY:
CHADL antibody is human, mouse and rat reactive. CHADL antibody is predicted to not cross-react with Chondroadherin protein.
Swissprot #:
Q6NUI6
User NOte:
Optimal dilutions for each application to be determined by the researcher.