JAGN1 Antibody

Contact us
ProSci
Product Code: PSI-8367
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8367-0.02mg0.02mg£150.00
Quantity:
PSI-8367-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
JAGN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

1 / 2
Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.
❮❯

Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
Jagunal homolog 1
Application Note:
JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1). JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1). Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2).
Background References:
  • Boztug K, Jarvinen PM, Salzer E, et al. JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia. Nat. Genet. 2014; 46:1021-7.
  • Wirnsberger G, Zwolanek F, Stadlmann J, et al. Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense. Nat. Genet. 2014; 46:1028-33.
Buffer:
JAGN1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1.

The immunogen is located within the last 50 amino acids of JAGN1.
NCBI Gene ID #:
84522
NCBI Official Name:
jagunal homolog 1
NCBI Official Symbol:
JAGN1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 20 kDa

Observed: 25 kDa
Protein Accession #:
NP_115881
Protein GI Number:
190014601
Purification:
JAGN1 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Immunology
SPECIFICITY:
JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist; this antibody will detect all but isoform 2.
Swissprot #:
Q8N5M9
User NOte:
Optimal dilutions for each application to be determined by the researcher.