E2F1 Antibody

ProSci
Product Code: PSI-43-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-183-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Drosophila (fruit fly)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-183 (0.5ug/ml) staining of the Drosophila Eye-Antennal Imaginal Disc. Data obtained from Dr. Nam-Sung Moon, McGill University, Montreal, Canada.

43-183 (0.5ug/ml) staining of the Drosophila Eye-Antennal Imaginal Disc. Data obtained from Dr. Nam-Sung Moon, McGill University, Montreal, Canada.

Further Information

Additional Names:
E2f, E2F transcription factor, Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, DmelCG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, CG6376-PA, CG6376-PB, CG6376-PC, E2-promoter binding factor, E2f-PA, E2f-PB, E2f-PC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Immunofluorescence:This antibody shows identical pattern in the Drosophila eye-antennal imaginal disc as was observed before with a different antibody (Moon et al, Mol Cell Biol. 2006 Oct;26(20):7601-15. PMID: 16954388). Recommended concentration: 0.3-1ug/ml
Background References:
  • Zielke N, Kim KJ, Tran V, Shibutani ST, Bravo MJ, Nagarajan S, van Straaten M, Woods B, von Dassow G, Rottig C, Lehner CF, Grewal SS, Duronio RJ, Edgar BA.Control of Drosophila endocycles by E2F and CRL4(CDT2).
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TKGLFQTPYRSARN
NCBI Gene ID #:
42550
NCBI Official Name:
E2F transcription factor 1
NCBI Official Symbol:
E2F1
NCBI Organism:
Drosophila melanogaster
Physical State:
Liquid
Protein Accession #:
NP_524437.2
Protein GI Number:
24648770
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
Reported isoforms represent identical protein: NP_524437.2; NP_732646.1; NP_732647.1
Swissprot #:
Q27368