DNAJC5G Antibody

ProSci
Product Code: PSI-43-206
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-206-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-206 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

43-206 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
DNAJC5G, DNAJ (Hsp40) homolog, subfamily C, member 5 gamma, CSP-gamma, FLJ40417, DNAJ homolog subfamily C member 5G, cysteine string protein-gamma, dnaJ homolog subfamily C member 5G, gamma cysteine string protein, gamma-CSP, gamma-cysteine string protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Testis lysates after 0.05ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.4kDa according to provisional NP_775921.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining primarily in spermatogonia and in cellular membrane. Recommended concentration, 5-10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DSGRKYQQNVQSQP
NCBI Gene ID #:
285126
NCBI Official Name:
DnaJ heat shock protein family (Hsp40) member C5 gamma
NCBI Official Symbol:
DNAJC5G
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
Protein Accession #:
NP_775921.1
Protein GI Number:
27735107
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
Swissprot #:
Q8N7S2