Uromodulin Antibody

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ProSci
Product Code: PSI-43-156
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-156-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-156 (0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-156 (0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
UMOD, uromodulin, ADMCKD2, FJHN, HNFJ, HNFJ1, MCKD2, THGP, THP, OTTHUMP00000162212, OTTHUMP00000162213, Tamm-Horsfall glycoprotein, tamm-Horsfall urinary glycoprotein, uromodulin (uromucoid, Tamm-Horsfall glycoprotein), uromucoid
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line HeLa (calculated MW of 69.8kDa according to NP_003352.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Williams et al, Hum Mol Genet. 2009 Aug 15;18(16):2963-74. PMID: 19465746). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Ahluwalia TS, Lindholm E, Groop L, Melander O.Uromodulin gene variant is associated with type 2 diabetic nephropathy.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: DRCPHTRDSTIQ
NCBI Gene ID #:
7369
NCBI Official Name:
uromodulin
NCBI Official Symbol:
UMOD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 80kDa
Protein Accession #:
NP_003352.2
Protein GI Number:
137116
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants represent identical protein: NP_001008390.1, NP_003352.2
Swissprot #:
P07911