ERBB2 / HER2 Antibody

ProSci
Product Code: PSI-43-559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-559-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-559 (4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

43-559 (4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Further Information

Additional Names:
ERBB2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, c-erb B2/neu protein, herstatin, metastatic lymph node gene 19 protein, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, proto-oncogene Neu, proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase erbB-2, tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration, 4-6ug/ml.
Background References:
  • Ahmad I, Patel R, Singh LB, Nixon C, Seywright M, Barnetson RJ, Brunton VG, Muller WJ, Edwards J, Sansom OJ, Leung HY.HER2 overcomes PTEN (loss)-induced senescence to cause aggressive prostate cancer.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: TLIDTNRSRACHP
NCBI Gene ID #:
2064
NCBI Official Name:
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
NCBI Official Symbol:
ERBB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004439.2, NP_001005862.1
Protein GI Number:
119533
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_004439.2; NP_001005862.1). The immunizing peptide represents part of the extracellular domain.
Swissprot #:
P04626