cytokeratin 19 Antibody

ProSci
Product Code: PSI-43-491
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-491-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-491 (0.03ug/ml) staining of Human Colon cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-491 (0.03ug/ml) staining of Human Colon cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
KRT19, keratin 19, CK19, K19, K1CS, 40-kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in Human cancer lysates of Breast, Pancreas and Colon (calculated MW of 44.1kDa according to NP_002267.2). Recommended concentration: 0.03-0.1ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as reporter antibody in combination with EB12360
Background References:
  • Alix-Panabi?res C, Vendrell JP, Slijper M, Pell? O, Barbotte E, Mercier G, Jacot W, Fabbro M, Pantel K.Full-length cytokeratin-19 is released by human tumor cells: a potential role in metastatic progression of breast cancer.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HTEQLQMSRSEVTD
NCBI Gene ID #:
3880
NCBI Official Name:
keratin 19
NCBI Official Symbol:
KRT19
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 43kDa
Protein Accession #:
NP_002267.2
Protein GI Number:
24234699
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
P08727