NFATC3 / NFAT4 Antibody

ProSci
Product Code: PSI-43-582
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-582-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-582 (0.03ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-582 (0.03ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3, NFAT4, NFATX, T cell transcription factor NFAT4, nuclear factor of activated T-cells c3 isoform IE-Xa, nuclear factor of activated T-cells, cytoplasmic 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 116kDa according to NP_775188.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Amasaki Y, Adachi S, Ishida Y, Iwata M, Arai N, Arai K, Miyatake S, A constitutively nuclear form of NFATx shows efficient transactivation activity and induces differentiation of CD4(+)CD8(+) T cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HQELDAHEDDLQIN
NCBI Gene ID #:
4775
NCBI Official Name:
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3?
NCBI Official Symbol:
NFATC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 150kDa
Protein Accession #:
NP_775188.1, NP_004546.1, NP_775186.1, NP_775187.1
Protein GI Number:
27886561
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Neuroscience,Signal Transduction,
SPECIFICITY:
This antibody is expected to recognize all four reported isoforms (NP_775188.1; NP_004546.1; NP_775186.1; NP_775187.1).
Swissprot #:
Q12968