Pro-EGF Antibody

ProSci
Product Code: PSI-43-550
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-550-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
43-550 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 2

43-550 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5ug/ml.
Background References:
  • Ara?jo AP, Costa BM, Pinto-Correia AL, Fragoso M, Ferreira P, Dinis-Ribeiro M, Costa S, Reis RM, Medeiros R.Association between EGF +61A/G polymorphism and gastric cancer in Caucasians.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: SRQERVCNIEKNVS
NCBI Gene ID #:
1950
NCBI Official Name:
epidermal growth factor
NCBI Official Symbol:
EGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 160kDa
Protein Accession #:
NP_001954.2, NP_001171601.1, NP_001171602.1
Protein GI Number:
251757262
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Obesity, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
Swissprot #:
P01133