CD47 Antibody

ProSci
Product Code: PSI-43-581
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-581-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-581 (0.3ug/ml) staining of Human Hippocampus (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-581 (0.3ug/ml) staining of Human Hippocampus (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CD47, CD47 molecule, IAP, MER6, OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic surface determinant protein OA3, integrin associ
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Hippocampus lysates and 60kDa in lysates of cell lines HeLa and Jurkat (calculated MW of 35.2kDa according to NP_001768.1). The observed molecular weights correspond to glycosylated forms. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Azcutia V, Stefanidakis M, Tsuboi N, Mayadas T, Croce KJ, Fukuda D, Aikawa M, Newton G, Luscinskas FW.Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivo.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SNQKTIQPPRK
NCBI Gene ID #:
961
NCBI Official Name:
CD47 molecule
NCBI Official Symbol:
CD47
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 50kDa
Protein Accession #:
NP_001768.1, NP_942088.1
Protein GI Number:
4502673
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Neuroscience,
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001768.1; NP_942088.1).
Swissprot #:
Q08722