CDC48 / YDL126C Antibody

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ProSci
Product Code: PSI-43-588
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-588-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-588 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-588 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Baek GH, Kim I, Rao H.The Cdc48 ATPase modulates the interaction between two proteolytic factors Ufd2 and Rad23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Saccharomyces cerevisiae S288c, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HTKNMKLADDVDLE
NCBI Gene ID #:
7415
NCBI Official Name:
valosin containing protein
NCBI Official Symbol:
CDC48
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_010157.1
Protein GI Number:
6094447
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
This antibody may cross react in many other species.
Swissprot #:
P55072