CD4 Antibody

ProSci
Product Code: PSI-43-654
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-654-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-654 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-654 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
CD4, CD4 molecule, CD4mut, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Spleen lysates (calculated MW of 31.3kDa according to NP_001181943.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Background References:
  • Bernstein HB, Plasterer MC, Schiff SE, Kitchen CM, Kitchen S, Zack JA.CD4 expression on activated NK cells: ligation of CD4 induces cytokine expression and cell migration.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KNKEVSVKRVTQDPK
NCBI Gene ID #:
920
NCBI Official Name:
CD4 molecule
NCBI Official Symbol:
CD4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_000607.1, NP_001181943.1
Protein GI Number:
10835167
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Immunology
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1).
Swissprot #:
P01730