ITGB2 / LFA-1 Antibody

ProSci
Product Code: PSI-43-607
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-607-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-607 (0.3ug/ml) staining of K562 (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-607 (0.3ug/ml) staining of K562 (A) and U937 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ITGB2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor), cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, complement receptor C3 beta-subunit, complement receptor C3 subunit beta, integrin beta chain, beta 2, integrin beta-2, leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in lysates of cell lines K562 and U937 (calculated MW of 84.8kDa according to NP_00202.2). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Bolomini-Vittori M, Montresor A, Giagulli C, Staunton D, Rossi B, Martinello M, Constantin G, Laudanna C.Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ETQEDHNGGQKQLS
NCBI Gene ID #:
3689
NCBI Official Name:
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
NCBI Official Symbol:
ITGB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_000202.2
Protein GI Number:
124056465
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
Reported variants represent identical protein: NP_001120963.1, NP_000202.2.
Swissprot #:
P05107