EDF1 Antibody

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ProSci
Product Code: PSI-43-408
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-408-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-408 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-408 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
EDF1, endothelial differentiation-related factor 1, EDF-1, MBF1, multiprotein bridging factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10ug/ml.
Background References:
  • Brendel C, Gelman L, Auwerx J.Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-NKQHSITKNTAKLDR
NCBI Gene ID #:
8721
NCBI Official Name:
endothelial differentiation-related factor 1
NCBI Official Symbol:
EDF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 17kDa
Protein Accession #:
NP_003783.1, NP_694880.1
Protein GI Number:
4503453
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription,Transcription
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1).
Swissprot #:
O60869