SPO11 Antibody

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ProSci
Product Code: PSI-43-410
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-410-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-410 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-410 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
SPO11, SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae), MGC39953, SPO11 meiotic protein covalently bound to DSB-like, SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like, meiotic recombination protein SPO11
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in Human Testis lysates (calculated MW of 40.4kDa according to NP_937998.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Buhler C, Borde V, Lichten MMapping meiotic single-strand DNA reveals a new landscape of DNA double-strand breaks in Saccharomyces cerevisiae
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-NTYATKRDIYYTDSQ
NCBI Gene ID #:
23626
NCBI Official Name:
SPO11 meiotic protein covalently bound to DSB
NCBI Official Symbol:
SPO11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 38kDa
Protein Accession #:
NP_036576.1, NP_937998.1
Protein GI Number:
6912680
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cell Cycle,Cancer Cell Cycle
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_036576.1; NP_937998.1)
Swissprot #:
Q9Y5K1