apolipoprotein D Antibody

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ProSci
Product Code: PSI-43-461
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-461-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-461 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-461 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
APOD, apolipoprotein D, apo-D
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney and Adrenal Gland. Recommended concentration: 5ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as capturer and as reporter antibody in combination with EB12282.
Background References:
  • Chen H, Li Y, Du J, Cao Y, Li X.Increased JNK1 activity contributes to the upregulation of ApoD in the apocrine secretory gland cells from axillary osmidrosis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: EIEKIPTTFENGRC
NCBI Gene ID #:
347
NCBI Official Name:
apolipoprotein D
NCBI Official Symbol:
APOD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 25kDa
Protein Accession #:
NP_001638.1
Protein GI Number:
114034
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience,Signal Transduction,Alzheimer's Disease Neuroscience Signal Transduction
Swissprot #:
P05090