SERPINB1 Antibody

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ProSci
Product Code: PSI-43-507
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-507-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-507 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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43-507 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SERPINB1, serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa bands observed in Human Placenta lysates (calculated MW of 42.7kDa according to NP_109591.1). The fainter 45kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5ug/ml.
Background References:
  • Cooley J, Sontag MK, Accurso FJ, Remold-O'Donnell E.SerpinB1 in cystic fibrosis airway fluids: quantity, molecular form and mechanism of elastase inhibition.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KTINQWVKGQTEGK
NCBI Gene ID #:
1992
NCBI Official Name:
serpin peptidase inhibitor, clade B (ovalbumin), member 1
NCBI Official Symbol:
SERPINB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 42-45kDa
Protein Accession #:
NP_109591.1
Protein GI Number:
13489087
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
P30740