POU2F2 / OCT2 Antibody

ProSci
Product Code: PSI-43-198
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-198-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-198 (0.5ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-198 (0.5ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
POU2F2, POU class 2 homeobox 2, OCT2, OTF2, Oct-2, POU domain, class 2, transcription factor 2, homeobox protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Human Thymus and Tonsil lysates (calculated MW of 49.5kDa according to NP_002689.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Duan H, Xiang H, Ma L, Boxer LM, Functional long-range interactions of the IgH 3' enhancers with the bcl-2 promoter region in t(14;18) lymphoma cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-SPTGPSTKIKAED
NCBI Gene ID #:
5452
NCBI Official Name:
POU class 2 homeobox 2
NCBI Official Symbol:
POU2F2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 55kDa
Protein Accession #:
NP_002689.1
Protein GI Number:
4505959
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Signal Transduction,Transcription,Immunology Signal Transduction Transcription
Swissprot #:
P09086-4