MAP2K2 / MEK2 Antibody

Contact us
ProSci
Product Code: PSI-43-442
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-442-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
❮❯

43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MAP2K2, mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, dual specificity mitogen-activated protein kinase kinase 2, mitogen-activated protein kinase kinase 2, p45
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kDa according to NP_109587.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Duhamel S, H?bert J, Gaboury L, Bouchard A, Simon R, Sauter G, Basik M, Meloche S.Sef downregulation by Ras causes MEK1/2 to become aberrantly nuclear localized leading to polyploidy and neoplastic transformation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DLKMLTNHTFIKRS
NCBI Gene ID #:
5605
NCBI Official Name:
mitogen-activated protein kinase kinase 2
NCBI Official Symbol:
MAP2K2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 48kDa
Protein Accession #:
NP_109587.1
Protein GI Number:
13489054
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Signal Transduction,Cancer Signal Transduction
Swissprot #:
P36507