HES4 Antibody

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ProSci
Product Code: PSI-43-464
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-464-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-464 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-464 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
HES4, hairy and enhancer of split 4 (Drosophila), bHLHb42, bHLH factor Hes4, class B basic helix-loop-helix protein 42, hHES4, transcription factor HES-4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 26.1kDa according to NP_001135939.1). Recommended concentration: 1-3ug/ml.
Background References:
  • El Yakoubi W, Borday C, Hamdache J, Parain K, Tran HT, Vleminckx K, Perron M, Locker M.Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RSAAEHRKVGSRP
NCBI Gene ID #:
57801
NCBI Official Name:
hes family bHLH transcription factor 4
NCBI Official Symbol:
HES4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 24kDa
Protein Accession #:
NP_001135939.1
Protein GI Number:
10863967
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience,Transcription,Neuroscience Transcription
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_001135939.1) only.
Swissprot #:
Q9HCC6