ATP5F1 Antibody

ProSci
Product Code: PSI-43-547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-547-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-547 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-547 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, PIG47, ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transport
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human Heart lysates (calculated MW of 28.9kDa according to NP_001679.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Background References:
  • Elston T, Wang H, Oster G.Energy transduction in ATP synthase.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SIQHIQNAIDTE
NCBI Gene ID #:
515
NCBI Official Name:
ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
NCBI Official Symbol:
ATP5F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 25kDa
Protein Accession #:
NP_001679.2
Protein GI Number:
21361565
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity, Signal Transduction
Swissprot #:
P24539