CSTF3 Antibody

ProSci
Product Code: PSI-43-260
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-260-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-260 (0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-260 (0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CSTF3, cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa, CSTF-77, MGC117398, MGC43001, MGC75122, CSTF 77 kDa subunit, cleavage stimulation factor subunit 3, cleavage stimulation factor subunit 3, isoform 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 82.9kDa according to NP_001317.1). The observed molecular weight corresponds to the expected size of 77kDa. Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, SLarge-scale mapping of human protein-protein interactions by mass spectrometry.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RETKGKLPSYKE
NCBI Gene ID #:
1479
NCBI Official Name:
cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
NCBI Official Symbol:
CSTF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 75kDa
Protein Accession #:
NP_001317.1
Protein GI Number:
4557495
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
RNA Binding RNA Binding
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_001317.1) only.
Swissprot #:
Q12996