ICAM1 Antibody

ProSci
Product Code: PSI-43-164
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-164-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-164 (2ug/ml) staining of HeLa (A) and Daudi (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-164 (2ug/ml) staining of HeLa (A) and Daudi (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147), and other commercial sources. Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
Background References:
  • Fu C, Tong C, Wang M, Gao Y, Zhang Y, L? S, Liang S, Dong C, Long M.Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: SNCPDGQSTAKT
NCBI Gene ID #:
3383
NCBI Official Name:
intercellular adhesion molecule 1
NCBI Official Symbol:
ICAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_000192.2
Protein GI Number:
68067956
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Immunology
SPECIFICITY:
The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.
Swissprot #:
P05362