p15INK4b isoform 2 Antibody

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ProSci
Product Code: PSI-43-343
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-343-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-343 (1ug/ml) staining of Human Gastrointestinal cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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43-343 (1ug/ml) staining of Human Gastrointestinal cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CDKN2B, cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4), CDK4I, INK4B, MTS2, P15, TP15, p15INK4b, CDK inhibitory protein, CDK4B inhibitor, MTS-2, cyclin-dependent kinase 4 inhibitor B, cyclin-dependent kinases 4 and 6 binding protein, multiple tumor suppressor 2, p14-INK4b, p14_CDK inhibitor, p14_INK4B, p15 CDK inhibitor, p15-INK4b, p15_INK4B
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 12+14kDa bands observed in Human Gastrointestinal cancer lysates (calculated MW of 8.1kDa according to NP_511042.1). The observed 14kDa cannot represent isoform 1 and therefore it most likely represents another posttranslational modification of isoform 2. Recommended concentration: 1-3ug/ml.
Background References:
  • Geyer CR.Strategies to re-express epigenetically silenced p15(INK4b) and p21(WAF1) genes in acute myeloid leukemia.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RQGARERGARP
NCBI Gene ID #:
1030
NCBI Official Name:
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
NCBI Official Symbol:
CDKN2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 12 + 14kDa
Protein Accession #:
NP_004927.2, NP_511042.1
Protein GI Number:
17981694
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Cancer,Cell Cycle,Apoptosis Cancer Cell Cycle
SPECIFICITY:
This antibody is expected to recognize isoform 2 (NP_511042.1) only.
Swissprot #:
P42772