CD3D isoform A Antibody

ProSci
Product Code: PSI-43-633
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-633-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-633 (1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-633 (1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CD3D, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, IMD19, T3D, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), OKT3, delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 20kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 18.9kDa according to NP_000723.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Gil J, Busto EM, Garcill?n B, Chean C, Garc?a-Rodr?guez MC, D?az-Alderete A, Navarro J, Rein? J, Menc?a A, Gurbindo D, Bel?ndez C, Gordillo I, Duchniewicz M, H?hne K, Garc?a-S?nchez F, Fern?ndez-Cruz E, L?pez-Granados E, Schamel WW, Moreno-Pelayo MA, ReciA leaky mutation in CD3D differentially affects αβ and γδ T cells and leads to a Tαβ-Tγδ+B+NK+ human SCID.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HETGRLSGAADTQ
NCBI Gene ID #:
915
NCBI Official Name:
CD3d molecule, delta (CD3-TCR complex)
NCBI Official Symbol:
CD3D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 20kDa
Protein Accession #:
NP_000723.1
Protein GI Number:
4502669
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
This antibody is expected to recognize reported isoform A (NP_000723.1) only.
Swissprot #:
P04234