DUOX1 Antibody

ProSci
Product Code: PSI-43-377
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-377-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
43-377 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

43-377 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
DUOX1, dual oxidase 1, LNOX1, MGC138840, MGC138841, NOXEF1, THOX1, NADPH thyroid oxidase 1, flavoprotein NADPH oxidase, large NOX 1, long NOX 1, nicotinamide adenine dinucleotide phosphate oxidase, thyroid oxidase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Testis lysates (calculated MW of 177kDa according to NP_059130.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.
Background References:
  • Harper RW, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H, Wu R.Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CSRSDIETELTPQR
NCBI Gene ID #:
53905
NCBI Official Name:
dual oxidase 1
NCBI Official Symbol:
DUOX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 170kDa
Protein Accession #:
NP_059130.2
Protein GI Number:
20149640
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
SPECIFICITY:
Reported variants represent identical protein: NP_787954.1, NP_059130.2. This antibody is not expected to cross-react with DUOX2.
Swissprot #:
Q9NRD9