perilipin 3 / TIP47 Antibody

ProSci
Product Code: PSI-43-202
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-202-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-202 (0.03ug/ml) staining of HeLa (lane 1) and Human Colon cancer (lane two) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-202 (0.03ug/ml) staining of HeLa (lane 1) and Human Colon cancer (lane two) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PLIN3, perilipin 3, M6PRBP1, MGC11117, MGC2012, PP17, TIP47, 47 kDa MPR-binding protein, cargo selection protein TIP47, mannose-6-phosphate receptor-binding protein 1, perilipin-3, placental protein 17, tail-interacting protein, 47 kD
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa+45kDa double bands observed in lysates of cell line HeLa and of Human Colon cancer (calculated MW of 47.1kDa according to NP_NP_005808.3, 46.9kDa according to NP_001157661.1 and 45.8kDa according to NP_001157666.1). The observed bands represent the three different isoforms. Please note the difference in band pattern intensities between HeLa and Colon cancer lysates. Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Hocsak E, Racz B, Szabo A, Mester L, Rapolti E, Pozsgai E, Javor S, Bellyei S, Gallyas F Jr, Sumegi B, Szigeti A.TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stress-induced cell death.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TRGAVQSGVDKTKS
NCBI Gene ID #:
10226
NCBI Official Name:
mannose-6-phosphate receptor binding protein 1
NCBI Official Symbol:
PLIN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 48kDa + 45kDa
Protein Accession #:
NP_005808.3, NP_001157661.1, NP_001157666.1
Protein GI Number:
68846601
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience,Signal Transduction,Neuroscience Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_005808.3; NP_001157661.1; NP_001157666.1).
Swissprot #:
O60664