MIA / CD-RAP Antibody

ProSci
Product Code: PSI-43-213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-213-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-213 (1ug/ml) staining of Human Glioma lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-213 (1ug/ml) staining of Human Glioma lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
MIA, melanoma inhibitory activity, CD-RAP, melanoma-derived growth regulatory protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 14kDa band observed in Human Glioma lysates (calculated MW of 14.5kDa according to NP_006524.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Skeletal Muscle. Recommended concentration: 5ug/ml.
Background References:
  • Hofmann MA, Schicke B, Fritsch A, Biesold S, Gussmann F, K?chler I, Voit C, Trefzer U.Impact of lymph node metastases on serum level of melanoma inhibitory activity in stage III melanoma patients.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PKLADRKLCADQE
NCBI Gene ID #:
8190
NCBI Official Name:
melanoma inhibitory activity
NCBI Official Symbol:
MIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 14kDa
Protein Accession #:
NP_006524.1
Protein GI Number:
2498559
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity
SPECIFICITY:
The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1,
Swissprot #:
Q16674