ICBP90 / UHRF1 Antibody

ProSci
Product Code: PSI-43-118
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-118-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-118 (2ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-118 (2ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
UHRF1, ubiquitin-like, containing PHD and RING finger domains, 1, Np95, ICBP90, FLJ21925, transcription factor ICBP90, RNF106, MGC138707, RING finger protein 106, inverted CCAAT box-binding protein of 90 kDa, nuclear zinc finger protein Np95, transcription factor ICBP90, ubiquitin-like with PHD and ring finger domains 1, hNP95, E3 ubiquitin-protein ligase UHRF1,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kDa according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer Res. 2000 Jan 1;60(1):121-8. PMID: 10646863). Recommended concentration: 1-3ug/ml.
Background References:
  • Hopfner R, Mousli M, Jeltsch JM, Voulgaris A, Lutz Y, Marin C, Bellocq JP, Oudet P, Bronner C. ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-LNQLFPGYGNGR
NCBI Gene ID #:
29128
NCBI Official Name:
ubiquitin-like with PHD and ring finger domains 1?
NCBI Official Symbol:
UHRF1
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_001041666.1, NP_037414.3
Protein GI Number:
115430235
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Signal Transduction
SPECIFICITY:
This antibody is expected to recognise both reported isoforms.
Swissprot #:
Q96T88