Transcription factor E2F1 Antibody

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ProSci
Product Code: PSI-43-415
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-415-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-415 (1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-415 (1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
E2F1, E2F transcription factor 1, E2F-1, RBAP1, RBBP3, RBP3, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9kDa according to NP_005216.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Hu F, Gartenhaus RB, Zhao XF, Fang HB, Minkove S, Poss DE, Rapoport AP.c-Myc and E2F1 drive PBK/TOPK expression in high-grade malignant lymphomas.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QEVTSEEENRATDS
NCBI Gene ID #:
1869
NCBI Official Name:
E2F transcription factor 1
NCBI Official Symbol:
E2F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_005216.1
Protein GI Number:
12669911
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Cancer,Cell Cycle,Transcription,Apoptosis Cancer Cell Cycle Transcription
Swissprot #:
Q01094