BCAR1 Antibody

ProSci
Product Code: PSI-43-375
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-375-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-375 (1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-375 (1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
BCAR1, breast cancer anti-estrogen resistance 1, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas scaffolding protein family member 1, Crk-associated substrate p130Cas, breast cancer anti-estrogen resistance protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml.
Background References:
  • Huang W, Deng B, Wang RW, Tan QY, He Y, Jiang YG, Zhou JH.BCAR1 protein plays important roles in carcinogenesis and predicts poor prognosis in non-small-cell lung cancer.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KQTSTFSKQTPHH
NCBI Gene ID #:
9564
NCBI Official Name:
breast cancer anti-estrogen resistance 1
NCBI Official Symbol:
BCAR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
Protein Accession #:
NP_001164185.1, NP_001164186.1, NP_001164187.1, NP_001164188.1, NP_001164189.1, NP_055382.2, NP_001164190.1, NP_001164191.1, NP_001164192.1
Protein GI Number:
44662836
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.
Swissprot #:
P56945