alpha-fetoprotein Antibody

ProSci
Product Code: PSI-43-556
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-556-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-556 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-556 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
AFP, alpha-fetoprotein, FETA, HPAFP, alpha-1-fetoprotein, alpha-fetoglobulin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line HepG2 (calculated MW of 68.7kDa according to NP_001125.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Hung TM, Hu RH, Ho CM, Chiu YL, Lee JL, Jeng YM, Shih DT, Lee PH.Downregulation of alpha-fetoprotein expression by LHX4: a critical role in hepatocarcinogenesis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KDALTAIEKPTGDEQ
NCBI Gene ID #:
174
NCBI Official Name:
alpha-fetoprotein
NCBI Official Symbol:
AFP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_001125.1
Protein GI Number:
4501989
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
Swissprot #:
P02771