GPI / Neuroleukin Antibody

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ProSci
Product Code: PSI-43-136
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-136-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-136 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-136 (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
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43-136 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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43-136 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-136 (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
43-136 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
GPI, glucose phosphate isomerase, AMF, GNPI, NLK, PGI, PHI, SA-36, autocrine motility factor, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, neuroleukin, oxoisomerase, phosphoglucose isomerase, phosphohexomutase, phosphohexose isomerase, phosphosaccharomutase, sperm antigen-36
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates and approx 55kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
Background References:
  • Iwanami K, Matsumoto I, Tanaka Y, Inoue A, Goto D, Ito S, Tsutsumi A, Sumida T, Arthritogenic T cell epitope in glucose-6-phosphate isomerase-induced arthritis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RERMFNGEKINYT
NCBI Gene ID #:
2821
NCBI Official Name:
glucose-6-phosphate isomerase
NCBI Official Symbol:
GPI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_000166.2
Protein GI Number:
18201905
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Neuroscience,Signal Transduction,Cancer Immunology Neuroscience Signal Transduction
Swissprot #:
P06744